I want to create this material at lab
I transfected Huh 7.5 cell lines with vector pDG461which harbors Cas9. I performed qPCR to make sure that Cas9 is expressed in my cell lines. I used GAPDH as a reference gene in qPCR and my...
11 July 2020 7,139 11 View
Hi everyone I am looking for a precise and comprehensive source for protocols of laboratory techniques which are used in biotechnology or cell culture and related subjects .Do you know guys any...
21 May 2020 889 8 View
Dear all, I have some bacteria DNA partial sequences (16s rRNA). They sequenced only by 9 F primer. Their length is under 1100 bp. When I draw phylogenetic trees for some of them by MEGA, my...
23 April 2020 9,203 8 View
I want to know origin of spectral shift in my plasmonic sensor when medium becomes more and more acidic.
08 October 2019 4,247 3 View
hello every one I am looking for a really precise protocol for doing protein SDS PAGE and also Western blot and of course a good source for how to deal with their trouble shooting . any kind of...
23 August 2019 2,104 5 View
I was reading the patent " Method of providing a substrate with a hydrophilic coating and substrates, particularly medical devices, provided with such coatings", and in one of the examples, it...
21 December 2018 4,397 3 View
Hi I am looking for a really functional source for learning more about details on the topic of Comparative Genomics .I would be appreciated to introduce me couple of them .
16 November 2018 2,647 9 View
Does anyone know if there is any formula I can use for calculation of a drug concentration (knowing oral dose) in small intestine and/or other parts of GI? Thanks
20 February 2018 2,352 1 View
It seems that these particles are formed in the shape of flakes with nanosize thickness.
15 May 2017 6,716 1 View
Please tell me what is aripiprazol?
04 January 2012 7,098 15 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View
Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...
22 February 2021 730 2 View
I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples
22 February 2021 1,328 1 View
I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...
22 February 2021 5,324 3 View
I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?
21 February 2021 2,558 5 View
Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...
21 February 2021 7,447 2 View
I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...
20 February 2021 5,820 4 View
Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...
18 February 2021 371 4 View
