I want to create this material at lab
I ran Process (Model 7) for my research and I found a significant interaction on the A path. I tested for the indirect effect and got a significant indirect effect, but the index of moderated...
15 June 2024 6,794 3 View
how can i have one peak in melting curve of primer in Real-time PCR?
06 May 2024 419 4 View
Hello Can anybody suggest me good C18 column for the separation of small molecules which has a long life?
06 February 2024 5,719 4 View
Hello, I have nylon fabric dyed with Acid dye. I need to prove for part of my project, is it possible or not possible, the reaction between dye molecules absorbed in nylon fabric with water...
01 February 2024 1,671 4 View
Hi all I’m working with RP HPLC With injecting the sample from the same vial on a 4.6x250mm column (C18, 5um, 1ml/min) for several injections I get varying peak integrity Is there a rational...
28 December 2023 9,239 12 View
I am writing to ask for more information about bioinformatics ideas for a humanized antibody. A humanized antibody has been causing me to wonder what kind of bioinformatician analysis I can do....
04 October 2021 6,961 3 View
I an undertaking tube formation matrigel assay using HUVEC cells. I've tried using 24 well plate and the images looked great! However, due to the expense of using matrigel I tried with a 48 well...
29 May 2021 1,177 2 View
I transfected Huh 7.5 cell lines with vector pDG461which harbors Cas9. I performed qPCR to make sure that Cas9 is expressed in my cell lines. I used GAPDH as a reference gene in qPCR and my...
11 July 2020 7,299 11 View
Hi everyone I am looking for a precise and comprehensive source for protocols of laboratory techniques which are used in biotechnology or cell culture and related subjects .Do you know guys any...
21 May 2020 1,064 8 View
Dear all, I have some bacteria DNA partial sequences (16s rRNA). They sequenced only by 9 F primer. Their length is under 1100 bp. When I draw phylogenetic trees for some of them by MEGA, my...
23 April 2020 9,358 8 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View
As mentioned in literature the addition of CaCO3 to MRS Agar media is considered as an indicator for lactic acid producing bacteria, so I wonder if we can add CaCO3 calcium carbonate to any other...
20 July 2024 7,504 0 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
15 July 2024 2,414 1 View
I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...
12 July 2024 3,756 4 View
