I'm trying to grow Enterococcus faecium in 96-well plates in BHI using a plate incubator at 35 °C, 100 rpm.The cells grow normally in tubes with BHI at 35 °C, but when I dilute them to the micro plates they stop growing. I fill 200 µL in ~370 µL wells.

Is this because of oxygen diffusion limitations? Any other reason?

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