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I'm trying to grow Enterococcus faecium in 96-well plates in BHI using a plate incubator at 35 °C, 100 rpm.The cells grow normally in tubes with BHI at 35 °C, but when I dilute them to the micro...
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In the bottle of a M17 medium they recommend to autoclave the medium without lactose, wait cool down to 50 °C and add (filter sterilized) lactose (or glucose). The medium (37.25 g) is composed...
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