Spheroid cancer cells highly express E-cadherin and membrane-typed beta-catenin due to the enhanced cell-to-cell adhesion. Depending on tumor types, spheroid formation leads to the up-regulation of E-cadherin as compared with 2D culture.   

You might try quantifying the E-cadherin transcription level with PCR since using trypsin to dissociate cells induces usually a considerable lose of cadherin. 

 Hi Rajan,

you could try to perform :

- some immunofluorescence staining and imaged them with a multi-photon microscope to look at the E-caderin localisation,

- and Western blot to look at expression level.