Hello ! I have a weak signal for Citrine attached to an AAV plasmid. I have heard that I can enhance the signal by using a GFP antibody. Any advice ?
Hi everyone, I am writing a research proposal for my masters applications and am in dire need of guidance. I suggest to quantitatively investigate the potential correlation between feminist...
05 February 2021 3,666 8 View
Dear all, good day, I am working with a virus expressing dsRed which I injected in mouse brain, in combinaison with another one expressing EYFP. The dsRed signal is too weak to be visible under...
05 May 2020 517 2 View
I am conducting a meta-analysis and most of my studies report values for bacterial analysis in CFU. However, I have one study which reports its microbial values in MPN. What should I do?
29 April 2020 662 5 View
Good day, We recently ran an experiment for c-fos staining within the amygdala. 90 minutes after the behavior, the animals have been transcardially perfused with PFA and the brains have been put...
08 March 2020 6,852 4 View
Hello, I am working with a convex hull in n-dimensions (n>3) and I am having problems generating points on the convex hull surface. Ideally, I would like the points to be uniformly distributed or...
06 January 2020 222 3 View
The module scipy.spatial.ConvexHull can only compute the Minkowski sum of two 2D convex hulls. But I would like to calculate the Minkowski sum of two 3D convex hulls. I am wondering if there is...
05 September 2018 2,887 3 View
Dear all, I am facing Subit issues, for mosquito genomic DNA measurements (for gDNA library preparation for NGS). The same sample was measured by 3 different people (in december 2015, august 2016,...
26 February 2017 2,726 5 View
We are facing a problem in different groups in our institute: when we grow E. coli (DH5a) overnight in liquid LB, we have: - no growth at all - sometimes, some tubes show cell growth, some...
20 February 2017 2,314 12 View
I have genomic DNA eluted in TE buffer, and am planning to sequence them. Unfortunately, EDTA should be avoided for Nextera library preparation. I am thus planning to purify my gDNA again, using...
19 March 2015 4,615 22 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View