Hello ! I have a weak signal for Citrine attached to an AAV plasmid. I have heard that I can enhance the signal by using a GFP antibody. Any advice ?
Is there any normalization of the genus abundance matrix during enterotyping using the Dirichlet multinomial mixture (DMM) method? If so, what is it? Thanks in advance.
07 March 2024 2,291 2 View
Hi ! I'd like to induce a DNA repair mechanism by homology with a donor sequence on mitochondrial DNA. Have you already succeeded in sending a template sequence to the mitochondria? If so, by what...
11 January 2024 1,364 2 View
Hi everyone, I am writing a research proposal for my masters applications and am in dire need of guidance. I suggest to quantitatively investigate the potential correlation between feminist...
05 February 2021 3,992 8 View
I have carried out a dietary questionnaire for the nutritional prevention of colorectal cancer, so I evaluate the amount of fiber consumed daily using a simple food frequency questionnaire. As...
08 December 2020 8,630 6 View
All in the question
09 August 2020 7,490 14 View
Good day, I prepared a K-Gluconate based internal solution which has a final osmolariry of 360 mOsm while I was expecting something around 300mOsm. It contains 170 K-gluconate, 10 HEPES,...
19 July 2020 751 0 View
Dear all, good day, I am working with a virus expressing dsRed which I injected in mouse brain, in combinaison with another one expressing EYFP. The dsRed signal is too weak to be visible under...
05 May 2020 715 2 View
I am conducting a meta-analysis and most of my studies report values for bacterial analysis in CFU. However, I have one study which reports its microbial values in MPN. What should I do?
29 April 2020 1,425 5 View
Good day, We recently ran an experiment for c-fos staining within the amygdala. 90 minutes after the behavior, the animals have been transcardially perfused with PFA and the brains have been put...
08 March 2020 7,025 4 View
Hello, I am working with a convex hull in n-dimensions (n>3) and I am having problems generating points on the convex hull surface. Ideally, I would like the points to be uniformly distributed or...
06 January 2020 403 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View