Yes, normalization of the genus abundance matrix is typically performed during enterotyping using the Dirichlet multinomial mixture (DMM) method. One common normalization technique is to scale the genus abundances to relative proportions within each sample.

Here's how the normalization process works:

By normalizing the genus abundance matrix, the DMM method can effectively account for differences in sequencing depth, sample composition, and technical variability, allowing for robust and accurate identification of microbial community structures and enterotypes.

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Thank you very much for your reply. If I understand correctly, normalization is not included in the DMN function. We need to perform normalization on the database beforehand. The DMN function requires a database with counts of OTU.

Regards,

Noémie Demaré