can any body suggest me any stabilizing agents to reduce protein aggregation and improve protein stability in solution at pH 7.5 ??
0.5 to 1 M trehalose
one strand - SSDNA - BIOTIN LABELLED another strand - SSDNA - NO LABEL without using NaCl , for Bli experiment ?
27 November 2023 9,035 0 View
How to Distinguish between DH5 alpha and BL21 cells if they are not labelled ?
25 August 2023 7,001 0 View
can you plese suggest other ways of restriction digestion of DNA.
19 June 2023 7,900 3 View
How can the size of protein (6kda) , dna (18bp, app. 6kda size ) and resultant complex around 12kda effects the change in the Fluorescence anisotropy ? Is there any correlation between the size...
01 April 2023 894 1 View
I am working on SSR markers.I have used Genodive software for analysis of tetraploid data. To check the genetic differentiation in 12 populations I applied AMOVA and the result file has attached....
11 April 2022 7,186 2 View
I don't know what happened. Suddenly I am unable to find my previous runs (DLS and zetapotential) data in my dts file. Even .del file was also not created which means data was not deleted. Anybody...
02 February 2022 9,008 5 View
can we use a palindromic sequence having dsDNA labelled with cyanine fluorophores for fret experiments ?
05 October 2021 5,629 1 View
y = A1*exp(-x/t1) + A2*exp(-x/t2)+y0
14 July 2021 7,668 3 View
What are the recommended buffers and ionic strengths for pH 10 , pH 12, pH 14, to study protein secondary structure in circular dichroism ?
04 June 2021 7,942 4 View
Can any one suggest me concentration of beta-mercaptoethanol (2-ME) or DTT to prevent cysteine dimerisation in protein purification at pH 8.0 . and stability of them in Tris Hcl buffer with...
16 December 2019 3,160 4 View
Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...
09 August 2024 5,444 7 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...
31 July 2024 9,697 0 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...
29 July 2024 9,753 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View