CCR7 together with CD45RA are commonly used to identify central memory, effector memory, EMRA, and naive T cells in humans by flow cytometry. However, in my current FACS panel I can't add CCR7. I can however add CD27 to the panel.
Some papers, such as: https://www.jimmunol.org/content/187/5/2093.long, use CD45RA together with CD27, however, it does not seem as common as CCR7 with CD45RA. Is there a known reason for that?
In general it seems CD27 provides improved separation and the epitope is not as affected by fixing.
Dear Dr. Sundling,
My understanding on memory subset markers is limited, but the article found in the below link might indicate that in the CD45RA- subset, we could detect CCR7-CD27+ memory T cells. So, this could affect the definition of the EM subset etc....
Article Stepwise Differentiation of CD4 Memory T Cells Defined by Ex...
Dear Dr.Sundling,
Using CD45RO, CD62L, HLADR and CD31 can clearly differentiate the 5 stages- Recent Thymic Emigrant (RTE) T cells, naive, central memory, Effector memory and terminally differentiated T cells. This was even highlighted in the recent Euroflow panel for Immunodeficiency disorders.
Dear Dr. Sundling
CD27 and CD45RA can be used to distinguish the populations that you mention. The Euroflow Primary Immunodeficiencies tube uses that strategy (link below).
Article The EuroFlow PID Orientation Tube for Flow Cytometric Diagno...
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