I would like to stain bone marrow stromal cells in rhesus macaques. I would appreciate suggestions on good FACS markers that you know work for human or rhesus stromal cells.
Bone Marrow Multipotential Stromal Cells (MSC) markers included CD105, CD 90 and CD73 molecules.
- CD105 is the TGF-beta receptor III, which potentially plays a role in TGF-beta signalling during MSC chondrogenic differentiation
- CD73 is an ecto-5'-nucleotidase, which is known to be involved in BM stromal interactions, MSC migration, and, potentially, MSC modulation of adaptive immunity.
The exact function of the CD90 (Thy1 antigen) is less well defined.
Great, thanks! I found a cross-reactive Ab for CD73, so I'll try with that.
May i know the marker which is positive for BMSC negative for macrophages.
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