Are there any differences between setting automatic or manual number of measurements in DLS?

When doing measurements in DLS (either size or zeta potential), I have the option to input an automatic number of measurements or to set it manually. My question is: is there a drawback in...

08 February 2021 9,423 6 View

Why IPNI voluntarily abbreviates some people's surnames in their standard forms?

The International Plant Name Index is known for standardising plant scientific names, including the standard forms of authors' names. I heard that one person's standard form may be chosen when...

01 January 2021 1,928 5 View

Google Reveals Major Hidden Weakness In Machine Learning, What are your observations on the following article?

Deep learning algorithms are prone to a previously unknown problem, say a team of computer scientists at...

14 December 2020 4,163 4 View

Which variables are related to transportation infrastructure?

I am conducting research on the "Impact of Transport Infrastructure on Economic Growth" Dependent Variable: GDP Growth (World Bank) Independent Variable : Railways, goods transported (million...

18 November 2020 9,014 6 View

Rounding decimals to use Poisson model?

I could not get a good fit for a Gaussian model of variation in the duration of a behavior. I thus rounded durations to the next integer (nearest minute) and used a Poisson distribution, which...

17 November 2020 4,048 4 View

Can you have a (very) local weather forecast model with simple weather stations?

Dear metereologists, sorry if this question is too basic, I'm just curious about it So, I`ve seen some personal stations that capture temperature, humidity, wind speed and pluviometry...

27 September 2020 178 4 View

How to avoid getting organic particles to charr(become black) during calcination?

I am trying to synthesize SrHfO3:Eu2+ nanoparticles. To reduce from Eu3+ to Eu2+, we calcinate it under Ar/H2 conditions but it ends giving a black coloured product. We need this for luminescence...

22 August 2020 448 3 View

Interpreting some regression coefficients of simple main effect and moderation analysis. How to interpret?

I did 2 regressions: Y = B0 +B1x1 + e and Y = B0 + B1x1 +B2x2 +B3x1*x2 + e (moderation) In the simple main effect, B1 is positive and significant. In the moderation analysis B1 become negative...

29 March 2020 8,855 3 View

Looking to increase your citation for sustainable tourism paper?

Dear Tourism Researchers, I am currently writing a book on Sustainable Tourism and if you have case studies or papers that you would like to share, I would love to provide citation to your work...

27 March 2020 8,298 3 View

How do I enter multiple points of I-V MOV characteristic from an external file in PSCAD?

I have a multiple points from a I-V MOV characteristic from a equipment datasheet, but PSCAD only allows to enter eleven points. I saw there is an option to enter until 100 points from an external...

28 February 2020 2,737 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View