Do you know evaluating methods for estimating GHG emissions and mitigation from farm ?

26 January 2017 5,094 9 View

Does the Unruh effect violate the cosmological principle?

Due to the Unruh effect the vacuum energy for the uniformly accelerated observer looks like as the equilibrium background with the Hawking-Unruh temperature $T=\hbar a/2\pi ck_B$, where $a$ is the...

01 June 2014 906 6 View

Can anyone help with the characteristic of chemical elements as indicators of human activities in the past (holocene, paleolithic etc.)?

Seeking insights into this question.

10 April 2014 1,557 4 View

Why in the Friedmann model can the expansion velocity of a receding galaxy be larger then the velocity of light?

Mathematical details for discussion see in arXiv:0810.0153

28 July 2012 149 19 View

Do You have some plans to evaluate of soil in cultural landscapes ?

01 January 1970 2,268 3 View

Are your samples completely homogeneous or will you somehow take into account their structure in depth?

As I remember, the proposed background correction just removes inelastic tail without any assumptions about surface structure of sample.

01 January 1970 8,086 3 View

How about Near-Infrared Spectroscopy to different age of SOM ?

SOM in paleosol and in burried soil have been the physical and chemical changes occurring in time. Which are capabilities of Near-Infrared Spectroscopy for assess the time dynamic of organic...

01 January 1970 864 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View