Hi,

Techniques have been developed for discriminating between human and mouse chromosomes, principally to aid the karyotypic analysis of human-mouse hybrids. These methods include fluorescent staining with Hoechst 33258, which causes mouse centromeres to fluoresce more brightly than human centromeres and alkaline staining with Giemsa. Below is the ppt link which may answer your question.

https://www.slideshare.net/varijnayan1/characterization-of-cell-line

 Hope this helps

 Short Tandem Repeat (STR) analysis. this compares your cell line to the original "parent tumor" and validates its authenticity and also karyotyping .

STR profiling of the cells