I just want to determine the TNF-α levels in human blood plasma and what is the most reliable, recent method for this?
theoretically it is possible. and my suggestion is to search these keywords together:
using Google: "the name of compound (gene) + Determination + HPLC "
I have found some genes that were determined by HPLC methods so it seems that similar procedure may be possible for your compound. and you should give more hints to the HPLC operator especially the polarity of compound and some solvents that suitable to be used as mobile phase. you should set the temperature of the column bellow the decomposition temperature of the compound and use a suitable flow rate.
Moreover try to use a MASS detector LC-MS chromatography system to help you understand the mass of each compounds that represent a peak in your obtained chromatogram.
Hope it helps.
The analysis to choose depends on the imprecision that is desired. For clinical studies the simplest option is the choice of an immunoenzymatic method (ELISA), easily susceptible to automation and with little cost of instrumentation.
If more specificity is desired, other more costly options should be considered.
references
Arican O, Aral M, Sasmaz S, Ciragil P. Serum Levels of TNF-α, IFN-γ, IL-6, IL-8, IL-12, IL-17, and IL-18 in Patients With Active Psoriasis and Correlation With Disease Severity. Mediators of Inflammation. 2005;2005(5):273-279. doi:10.1155/MI.2005.273.
Silvestri M, Bontempelli M, Giacomelli M, Malerba M, Rossi GA, Di Stefano A, Rossi A, Ricciardolo FL.High serum levels of tumour necrosis factor-alpha and interleukin-8 in severe asthma: markers of systemic inflammation?. Clin Exp Allergy. 2006 Nov;36(11):1373-81.
Lavanya Joshi, et al. Evaluation of TNF-α, IL-10 and IL-6 Cytokine Production and Their Correlation with Genotype Variants amongst Tuberculosis Patients and Their Household Contacts. PLOS Published: September 11, 2015
http://dx.doi.org/10.1371/journal.pone.0137727
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0137727
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