I used ATPase (ab7671) and ATPase (ab2825) with both mcf7 cells and human skin fibroblast cells, got nice staining in MCF7 cells but these two markers did not work with human skin fibroblast cells.
Na/K-ATPase is present in virtually any living animal cell, even erys.
One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...
08 August 2024 1,366 5 View
Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)
06 August 2024 6,610 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Nothing
01 August 2024 755 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
In a research, lesion diameter due to a disease has a CV below 10%. However, in an another parameter, disease inhibition percentage (compared to control), a value derived from lesion diameter...
27 July 2024 201 0 View
Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...
24 July 2024 3,284 6 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View
How to determine tannin content in fodder pellets?
22 July 2024 8,163 3 View
I want researchers who specialize in studying artificial intelligence in the field of science education and those related to it to give me information about the application of the...
18 July 2024 8,320 0 View
I am trouble shooting a malachite green ATPase assay using a kit from Sigma (MAK307) for a cytosolic protein that converts ATP --> ADP + Pi and am wondering what cheap + commercially available...
30 March 2024 9,659 1 View
I am trying to establish a PK/LDH system to detect ATP turnover. We are having difficulty observing any measurable change in NADH fluorescence (we are not using the absorbance approach), and...
16 January 2024 2,680 1 View
Does anyone has the profile of E coli. ATPase complex (F1Fo) size-exclusion chromatography (using a Superose 6 column), I mean what the profile looks like, how many peaks? Are they all well separated?
28 December 2023 1,278 0 View
Hello, During skeletal muscle contraction and during the actin–myosin ATPase cycle there is release of Pi and ADP. Can anyone tell me and ideally suggest a reading list of where they actually...
14 November 2023 6,583 4 View
Hello. Recently, I want to inject the adenosine in vivo to see its effect on the behaviors. But I am not sure whether it can cross blood-brain barrier. If it could, I would choose easier...
27 October 2023 5,687 2 View
What does it mean that it could have precipitated? What does it mean that NADH solution can be unstable? In some instances the absorbance increased from before to after I mixed in the...
12 April 2023 9,978 0 View
I'm doing ATPase activity assays, and trying to minimize free phosphates in the environment. I want to be able to deep clean a set of micropipettes and remove the phosphates from them. Does anyone...
10 November 2022 1,653 5 View
I am trying to figure out the best method to prepare vesicles of MDR1 (Pgp) for an ATPase assay and do not know the best method to use for preparing these vesicles. They have been growing in...
23 March 2022 8,109 1 View
For my project I have to pretreat RAW 264.7 macrophages with adenosine or 2-chloroadenosine for 1h and 24h, then followed with LPS (1ug/mL) treatment for another 24h. However, I'm not sure whether...
15 March 2022 4,004 2 View
I tried to use this kit but I found some problem with the bck groud control because they are too high if compare with my sample. Maybe something wrong with the endogenous phophate...
20 February 2022 4,296 0 View