Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

Please suggest the website or link for the collection of data for (Cancer Incidance Rates in India)?

We are doing research for the prediction of cancer incidance rates increases in India upto next five years. Please help us to get the exact data for Cancer Incidance Rates in India. This will...

19 February 2021 8,478 2 View

RDF calculation of water inside the Carbon nanotubes?

I am trying to calculate the rdf of water molecules present inside the Carbon nanotube. I tried to do the indexing first using gromacs tool gmx selection. But when it comes to calculating rdf, I...

17 February 2021 1,066 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What is the mechanical advantage of the K+-circulating system of cochlea? I thought simple ATPase structure would be enough to maintain [K+]. ?

Cochlea has a highly sophisticated system to maintain K+ concentration in the endolymph and IHC. I thought adopting Na+/K+ ATPase(just exchanging K+ mutually, directly) would be a lot simpler to...

14 December 2020 9,240 1 View

a Ferrari and an Oldsmobile with the same function?

I apologize for the analogy if you are really interested in cars. This is about protein function, but a crucial one because the extraction from membranes of a mistargeted protein is an essential...

15 October 2020 6,147 3 View

ADP-Glo test for ATPase activity of NLRP3?

Hello colleagues! I want to investigate the atphase activity of nlrp3 in the ADP-glo test. but I don't know what type of buffer to use for analysis. Can anyone help me?

12 September 2020 7,596 3 View

Is ATP the best energy providing molecule? can there be something better?

ATP energy-storing molecule as is no doubt an efficient! as not only it is used as energy storage and provider in all forms of life and has probably been around for billions of years. Even in the...

08 February 2020 9,015 6 View

What is the protocol that I can follow to join two RNA aptamers?

Currently I have two RNA aptamers, I read couple of papers that I can link 2 aptamers by poly adenosine linker but I do not know how to do that. If you have other suggestions of how to link two...

09 October 2019 9,327 2 View

Could someone recommend good antibodies for detecting M1, M3 AchR and SERCA1/2/3 in WB and IHC?

I'm working in a project related to Ca2+ signalling and I've to detect protein expression of M1/M3 Acetylcholine Receptors and SERCA1/2/3. Could someone recommend good antibodies for detecting...

08 September 2019 4,123 2 View

Can anyone recommend a Luminometer for ATPase assays?

We are currently in the market for a Luminometer tom perform the ATPase assay more efficiently. Does anyone have any recommendations with regards to model?

04 May 2019 4,132 2 View

Does anyone have a working metachromatic ATPase staining protocol in mouse skeletal muscle tissue?

I have tried the Ogilvie protocol with prestain buffer at pH 4.3 and 4.5 but the fibers are either all light blue or dark blue respectively. I've come across many papers which claim to use this...

03 April 2019 3,200 4 View

Can anybody help me to do the detailed calculation for the ATPase activity???

I am doing Na K ATPase activity assay by using the endpoint method, All goes well but now i am stuck in calculations. can anybody help me to make a detailed calculations. Thanks in advance

11 December 2018 6,438 2 View