What's the difference between 0.1% triton-X100 and ice-cold methanol in the immunofluorescence assay for intracellular protein staining?

Hi, Guys, I noticed that researchers usually permeabilized the cells with 0.1% Triton-X100 or ice-cold methanol (90%) for an intracellular protein staining, it looks like that the two methods work...

15 February 2021 5,606 4 View

How to effectively limit the output range of the intermediate layer of the cascaded neural network used in the reverse design of metamaterial?

The use of cascaded neural networks for the reverse design of metamaterials and nanophotons can effectively alleviate the problems caused by one-to-many mapping, but the intermediate layer of the...

05 August 2020 2,612 1 View

Is the existing Quantum Computer able to break the current 2020 DLT/Blockchain encryption ?

According to Google, it can achieve a quantum dominance with 53 qubits, i.e. 253 calculation simultaneously. However, this is not sufficient to break SHA2562 encryption through brute force with...

31 July 2020 2,424 3 View

Sticky black, irregular patches in cell culture flask?

Hi all, Recently I encountered some irregular black/grey patches inside my cell culture flask (see attached image, x400 magnification). They are not motile, not floating, do not stick to cells...

08 July 2020 7,921 3 View

Transfected cells died after trypsinization ?

Hi all, I am facing a problem to trypsinize and combine/move the transfected cells into new well . I was planning to pool the transfected cells into a new well to ensure better cell-cell...

21 December 2019 9,692 5 View

Problem with stable cell line generation?

Hi all, I am facing a problem to generate a stable cell line after successful transfection. I added puromycin to kill the untransfected cells, however, eventually all my transfected cells are...

19 September 2019 2,970 2 View

How to convert the arraystar circRNA number to circbase?

Several studies only showed the circRNA name from arrarystar, eg.mmu_circRNA_22066, without providing the spliced sequence or its position on chromosome. It will be hard for others to follow their...

20 August 2019 2,206 2 View

Co-transfection of miRNA mimics and inhibitors?

Hi, Is there any paper which reported the co-transfection of miRNA mimics and inhibitors? Thank you.

17 June 2019 1,087 3 View

MiRNA primer sequence?

Dear all, I have a miRNA primer sequence left by someone else and I would like to know about the sequence of this primer. Is there any method I can use to sequence and know the sequence of this...

14 June 2019 5,304 6 View

Qiagen or ThermoFisher Lipofectamine Transfection Reagent?

Hi all, I am planning for some transfection work involving a mammalian cancer cell line. Can I know the transfection kit from Qiagen or ThermoFisher lipofectamine is a better option? Do you...

23 March 2019 9,831 3 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

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01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View