What I knows is Tris buffer interferes in cross linking of DNA binding proteins complex using glutaraldehyde. My oligos (dodecamer) are suspended in Tris pH 7.5. If I want to remove Tris from the oligos solution, what is the best feasible way that I can proceed with..
In my mind Dialysis could be another way to solve the problem. But I don't know as for the oligos...
Is there anyone who has came across this issue????
Desalting through a small sephadex g25 column works well and quickly. Oligos are excluded from the matrix and come out quickly but salts run through the entire column and come off later in the total column volume. Samples can be run under gravity or spun through in a centrifuge. This method is also used to clean up small molecules and salts in sequencing reactions and is well tested
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