Johnson Luwang Wahengbam

3 Questions 2 Answers 0 Followers

Questions related from Johnson Luwang Wahengbam

"Protein Transition State" existence in gel filtration chromatography?

Has anyone observed any oligomeric protein(s) captured in transition state between the two oligomeric state of the protein in Gel filtration chromatography? The protein has history of dimer and...

01 December 2017 1,974 3 View

DNA tagged Cy3 or DNA tagged Cy5 dye! Which one is better for (EMSA) Electrophoretic Mobility Shift Assay studies?

Dear All,I am planning to study Protein-DNA interaction in my research work. What I am planning to do is to carry out EMSA experiment but non radio labelled one! So, what comes in my mind is to...

09 May 2016 9,484 4 View

Can anyone suggest me the best way to remove the Tris buffer from the dodecamer Oligos suspended in Tris pH 7.5 buffer?

What I knows is Tris buffer interferes in cross linking of DNA binding proteins complex using glutaraldehyde. My oligos (dodecamer) are suspended in Tris pH 7.5. If I want to remove Tris from the...

05 May 2016 2,858 2 View