I want to know activity of glycerate kinase from my microbial culture. I am not able to find out suitable method to do it properly. Please suggest me better protocol for analysis of this enzyme.
Hi there,
Glycerate kinase activity can be easily coupled to pyruvate kinase and lactate deshydrogenase. GK activity will result in the stoechiometric consumption of NADH measured at 340nm. For more info this paper should help :
http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2006.00264.x/full
Hi there,
Glycerate kinase activity can be easily coupled to pyruvate kinase and lactate deshydrogenase. GK activity will result in the stoechiometric consumption of NADH measured at 340nm. For more info this paper should help :
http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2006.00264.x/full
You can please refer to the links below to obtain certain interesting informations:-
1. http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2006.00264.x/full
2. http://www.sciencedirect.com/science/article/pii/S001457930600069X
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