I am working on the Bacillus sp. KK-1 xylanase gene and I want to find out this enzymes signal peptide. But this gene reportedly not contains any signal peptide. Please give me any reference or analytical software to deduce its signal peptide.
I would like to suggest svmsignal, phobius and signalp.
However, If the annotation of your gene comes from Uniprot's prediction, then it comes from phobius or signalp also. (http://www.uniprot.org/help/signal)
SignalP is one of the best tools. However, I suggest you reach consensus from the aforementioned suggested tools. In Bioinformatics, let us not rely on just one.