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I want to know activity of glycerate kinase from my microbial culture. I am not able to find out suitable method to do it properly. Please suggest me better protocol for analysis of this enzyme.
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I am working on the Bacillus sp. KK-1 xylanase gene and I want to find out this enzymes signal peptide. But this gene reportedly not contains any signal peptide. Please give me any reference or...
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I want to produce proteins at large scale fermenter level in Bacillus sp..Please anyone tell me which strain and vector I should use for large scale fermentation of industrially important enzymes...
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Dear all, I want to express pectin lyase D gene 1122bp (A. niger) in pichia pastoris X-33 using PpicZalpha A vector. In silico analysis showing translation of whole sequence in to amino acids,...
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I have amplified glucose oxidase gene (1.8 kb) from A. niger and used XhoI restriction site for forward primer and XbaI restriction site for reverse primer and successfully cloned in vector...
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