Ahmed Shah

5 Questions 16 Answers 0 Followers

Questions related from Ahmed Shah

Can anyone suggest me spectrometeric analysis of Bacterial glycerate Kinase?

I want to know activity of glycerate kinase from my microbial culture. I am not able to find out suitable method to do it properly. Please suggest me better protocol for analysis of this enzyme.

10 October 2015 1,081 4 View

How can I predict the signal sequence of the Enzyme Xylanase?

I am working on the Bacillus sp. KK-1 xylanase gene and I want to find out this enzymes signal peptide. But this gene reportedly not contains any signal peptide. Please give me any reference or...

12 December 2014 3,236 4 View

Which vector and strain of Bacillus sp. is most suitable for large scale fermentation of enzymes & proteins?

I want to produce proteins at large scale fermenter level in Bacillus sp..Please anyone tell me which strain and vector I should use for large scale fermentation of industrially important enzymes...

07 July 2014 3,356 1 View

Any advice on a problem in production of Pectin Lyase D (1122bp) from A. niger in Pichia pastoris?

Dear all, I want to express pectin lyase D gene 1122bp (A. niger) in pichia pastoris X-33 using PpicZalpha A vector. In silico analysis showing translation of whole sequence in to amino acids,...

03 March 2014 830 2 View

What could cause losing restriction sites for gene in our clone?

I have amplified glucose oxidase gene (1.8 kb) from A. niger and used XhoI restriction site for forward primer and XbaI restriction site for reverse primer and successfully cloned in vector...

12 December 2013 4,074 2 View