As i am using 500ml of RPMI + penicillin1% + FBS 10 % .
Hi Tahira,
In our experiments, SNU-251 cells purchased from the American Type Culture Collection, were cultured in RMPI 1640 medium supplemented with 10% FBS and antibiotics at 37°C in a 5% CO2 incubator. So, your medium content looks OK.
Best wishes,
Ilya
hi Serag. Can u provide me the reference for using this media ? i have not seen this media in contents.
Thanks.
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