How should I deal with missing data?

12 February 2021 5,306 4 View

No title

01 January 2021 9,970 2 View

How can I normalise cell count/protein concentration using SDS Coommasie blue stained gel?

Im quite new to cell culture and have had some inaccuracies while harvesting my cells and therefore counting them using a CASY counter. I reconstituted in what I thought to be 50,000 cells/ul,...

16 November 2020 9,829 1 View

Hazard value for malaria survival graph??

In an experiment untreated infected mice and infected treated mice observed. I recorded my data in terms of survived mice to 28 days in each group. How to calculate hazard value for treatment vs...

06 November 2020 5,548 1 View

What is the best blocking agent to block rGO biosensors?

I am trying to make a DNA sensor but I am detecting non specific binding. Does anyone know a better blocking agent for DNA sensors based on rGO, apart from BSA or MCH?

05 November 2020 5,140 3 View

No proteins of interest after Co-IP with Dynabeads?

Hello, I am trying to Co-IP my protein complex of interest, and every time i run it on the gel I get nothing but antibody bands. I conjugate my antibody to Dynabeads protein G overnight. I then...

29 October 2020 3,229 7 View

What kind of MMP should I use If I want to degrade rat tail collagen (Type I) gels?

I want to do MMP digestion on my collagen gels to see the effect of glycation on my collagen gels. I have difficulty to find the best MMP! some articles used MMP-1 some MMP-9 some MMP -2 and some...

28 July 2020 6,711 1 View

How can i test the Security for an algorithm in the field of IoT for a paper?

I need to know how to test the security of an implemented algorithm in the field of the IoT to state the test results in a paper. #IoT #Security #Blockchain #remix #Smart_Contract

01 July 2020 7,390 11 View

How to run Difference in Difference Different Time Periods?

Dear All, I have Panel Data fits difference in difference I regress the (Bilateral Investment Treaties-BIT) on (Bilateral FDI). BIT: is dummy taking 1 if BIT exists and Zero Otherwise....

29 May 2020 9,871 6 View

Thermodynamic properties of of the components of a certain solar thermophotovoltaic?

I am trying to simulate a solar TPV with a '1D Si/SiO2 PhC Emitter' and a single diode 'InGaAsSb pv cell'. now I am looking for 'thermal conductivity', 'Specific heat capacity' and 'density' of...

25 May 2020 7,818 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View