How do I interpret fit statistics SEM and Modindices output?

Hi, I would need some help in understanding the SEM output. I run a latent change score analysis to test the change score of a variable of interest at two-time points (repeated measure) PLD1 and...

05 January 2021 3,367 2 View

Lipofectamine 3000 in 10 cm dish?

Dear all, I'm trying to transfect HeLa cells with lipofectamine 3000 in a 10 cm dish. Do someone of you have a protocol to share? I still examined the table for scaling up or down lipo3000 but it...

13 October 2020 2,926 3 View

Which is the best system to keep endocrinology samples frozen in the field, without electricity?

Hi everyone, I am looking for suggestions to store biological samples in the field for endocrinology analyses, where I will not have electricity. One of the options we are evaluating is a solar...

01 September 2020 3,766 3 View

How to combine beam and shell elements in ANSYS APDL?

Hi everyone! I have a doubt and I hope there is someone who can give me an advice. I am modeling a MEMS gyroscope for my Master thesis and I am starting with a very basic structural design. My...

20 August 2020 9,524 5 View

How to edit density and heat capacity in Aspen Plus ?

Recently I'm trying to edit a pure component mass density and heat capacity of an existing component NaNO3 but failed. Could anyone help me on this? Thanks a lot.

17 July 2020 8,800 8 View

Do you have suggestions about CROSS CULTURAL ADAPTATION of questionnaires?

Hi! I am currently translating and adaptating a questionnarire about attitudes towards inclusion, and I would like to have suggestions about specific literature in translation and cross-cultural...

23 March 2020 2,630 3 View

Can the pcDNA5FRT/TO vector be used for transient overexpression of a gene in a common cell line?

01 March 2020 3,206 2 View

Does CXCL8 or other chemokines induce in vitro cell proliferation?

hi everybody, i have some troubles with chemokines and cell proliferation. Different papers evidence that CXCL8 (but also CXCL12, CCL2 and other chemokines) are able to promote cancer cell...

03 February 2020 214 4 View

Anyone could suggest how to analyze calcium imaging using imagej?

Hi, we would like to analyze time sequences images of calcium imaging assay performed with Fluo4-AM in imagej. Is there any plugin that we could install to do this? Do you have any kind of...

27 January 2020 2,665 1 View

Chloroform and isopropanol RNA extractions?

I have to perform RNA extraction with TRIzol LS and I just realized that we have chloroform stabilized with ethanol. Is that ok? And should chloroform and isopropanol be "molecular biology...

30 September 2019 4,647 5 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View

For PC-3 cell line. How long before irradiation do I have to synchronize my cells to G0/G1?

I am trying to do a clonogenic assay for PC-3 after irradiation. I have to synchronize my cells by serum starvation but there's a optimal time I have to do that before irradiation. been looking in...

09 February 2021 9,480 1 View