Can anyone suggest an ultracentrifugation procedure for isolating exosomes from cell culture medium?

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Adrian Herrera

Hi Nilufer,

I work at Immunostep, a biotech company that developed a full product line dedicated to exosomes due to their potential. Maybe I can help you from my experience.

I would recommend you to check our website, specifically the ExoStep one: https://immunostep.com/exosomes/exostep-platform-detection-and-characterization/

Furthermore, you will find attached in this message the Technical Data Sheet of the ExoStep Cell Culture, that has a part dedicated to the Protocol of Use.

I think that it will help you and clarify a lot of questions.

Do not hesitate to ask if you have any doubt.

Neha Rana

Hi, below are 2 articles with UC protocols you could try :

1. https://star-protocols.cell.com/protocols/440

2. Article Exosome Isolation by Ultracentrifugation and Precipitation a...

Regards

Neha

Zach Wang

Here is some protocols and overview of different ultracentrifugation methods: http://assets.thermofisher.com/TFS-Assets/LPD/Application-Notes/protocol-cell-culture-exosome-whitepaper.pdf

https://lab.plygenind.com/methods-to-isolate-exosomes