I’m having trouble to transfect CH12F3 cells. I tried lipofection using lipofectamine, it didn’t work. We have a biorad gene pulser xcell and I’m thinking to do an Electroporation.
Hello
Kindly look at in these links
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2975437/
http://www.jimmunol.org/content/jimmunol/early/2013/07/10/jimmunol.1300922.full.pdf
Good Luck
There's a nice collection of protocols available at http://biorad-ads.com/transfection_protocols/
Best,
C
Thank you all
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