Article Human Saliva-Derived Exosomes

Ultracentrifugation to separate exosomes

The pooled saliva is centrifuged at 12,000 × g for 20 min to remove residual organelles and cell fragments. Following this, 0.5 ml supernatant is diluted 1:1 with phosphate-buffered saline (PBS) and transferred to 1.0 ml polycarbonate tubes for ultracentrifugation at 120,000 × g for 3 hr at 4C The final pellet is resuspended in 100 µl PBS and then kept at -70C until further analysis.

Bhairavi Vajaria thank you. Is there a way of doing it without the need to use ultracentrifugation?

Please refer this article:

Article A Comparison of Traditional and Novel Methods for the Separa...

several ultracentrifugation protocols were published for saliva. but this sample is rather challenging, I'd advise to use Total exosome isolation reagent (Invitrogen)

Thank you Alexander Sasha Vlassov

Without a kit, you should probably do ultracentrifugation.

However, it also seems you wish to avoid the ultracentrifugation process, then you can isolate microvesicles containing exosomes by 10,000g after filtration: this step is called exosomes in media and not the concentrated/enriched exosomes.

Thanks Abayomi Opadele