Nur Basirah Ghazali

4 Questions 7 Answers 0 Followers

Questions related from Nur Basirah Ghazali

Why is my amplification plot looking like this?

Hello, I'm at the stage of optimizing my qRT-PCR protocol. Can anyone tell me why my plot looks like this? I'm using Taqman advanced microRNA assay and universal PCR mastermix no amperase...

15 August 2020 5,683 3 View

What can be used as the loading control for western blot using saliva samples other than amylase?

Hi! I know people use beta-actin and GAPDH as loading control for tissue samples and cells, but other than amylase, what else can I use for saliva samples? Can I use sIgA? I've used the antibody...

19 August 2015 4,561 1 View

What is the best method of extracting protein from saliva samples?

Hi. I am currently doing western blot experiments using saliva samples. I'm trying out different methods of extraction of the proteins in saliva. I tried extraction with ethanol. It worked fine, I...

13 August 2015 9,309 9 View

Are you familiar with simple experiments that can show an increase in phosphate?

We're conducting an experiment to develop an assay to measure phosphate. At this stage we need a simple experiment that we can do to show an increase in phosphate. Do you have any suggestions?

19 April 2015 8,217 4 View