Nur Basirah Ghazali

6 Questions 12 Answers 0 Followers

Questions related from Nur Basirah Ghazali

Why is my amplification plot looking like this?

Hello, I'm at the stage of optimizing my qRT-PCR protocol. Can anyone tell me why my plot looks like this? I'm using Taqman advanced microRNA assay and universal PCR mastermix no amperase...

15 August 2020 5,691 3 View

Why is it difficult to detect IL-1 beta in serum compared to in saliva?

I'm new in the area and would like to know what makes IL-1 beta easier to detect in saliva as compared to the blood.

15 August 2017 3,280 2 View

Is the ratio of pro-IL-1beta to cleaved IL-1beta unique to each individual?

I've been trying to look at the pattern of both full-length and cleaved il-1beta forms. Is it possible that all individuals (healthy) have the same ratio of full-length to cleaved forms or...

17 November 2015 3,194 4 View

What can be used as the loading control for western blot using saliva samples other than amylase?

Hi! I know people use beta-actin and GAPDH as loading control for tissue samples and cells, but other than amylase, what else can I use for saliva samples? Can I use sIgA? I've used the antibody...

19 August 2015 4,572 1 View

What is the best method of extracting protein from saliva samples?

Hi. I am currently doing western blot experiments using saliva samples. I'm trying out different methods of extraction of the proteins in saliva. I tried extraction with ethanol. It worked fine, I...

13 August 2015 9,323 9 View

Are you familiar with simple experiments that can show an increase in phosphate?

We're conducting an experiment to develop an assay to measure phosphate. At this stage we need a simple experiment that we can do to show an increase in phosphate. Do you have any suggestions?

19 April 2015 8,234 4 View