Dear Amlan:

You can determine the size distribution of nanoparticles by your DLS. To this should be used with the exact dispersant and viscosity, index of refraction, such as water or individual hydrocarbon. The dispersion should be stable and diluted . Periodic method. You can not measure the size of the nanoparticles in the process flow. See www.malvern.com

Regards

Hi Yuri,

I think you did not get my question. I am using the very same conditions as you said. What I want is a protocol that uses a co-precipitation method for the synthesis and generates particles less than 40 nm with a narrow size distribution. The analytical method for size determination would be DLS. Im using the Malvern Zetasizer.

Amlan:

Dissolve 2.8 g FeSO4∙7H2O in 150 см3 of water, 5.4 g FeCl3∙6 H2O in 150 см3 of water and 50 g NaOH in 50 см3 of water. Combine the first two solutions ( all 300 см3). Мake seperate solutions and then combine. The resulting solution was stirred and add slowly with stirred of alkaline solution. Without nitrogen atmosphere and increase temperature. The resulting black precipitate magnetite washed to pH = 7. Transfer the precipitate to a porcelain cup, add 50-100 см3 of 0.01 kmol / m3 HCl. Thoroughly dispersed. Can sonicate.The resulting sol magnetite (6-8 nm) was filtered through a membrane filter.

Regards

Hi Yuri,

Thank you so much for the protocol. I was looking for something like this. Just to be clear. Are you telling me to make one 150 ml solution in which Im to dissolvle 2.8 g FeSO4∙7H2O and 5.4 g FeCl3∙6 H2O or make seperate solutions and then combine? Secondly isnt 50g NaOH for 50 ml water too concentrated? Did you mean 5g in 50 ml?

Thank you again.

Plus Yuri, should I be using a nitrogen atmosphere during the stirring? Also should I increase the reaction temperature to 90 degrees C.? Or you say to directly do it without any such conditions.

Hi Sonja,

Thanks for your answer, I know TEM is a good option always but I have other things associated with the SPIONs so the hydrodynamic diameter is the one that Im actually looking for.