Amlan Chakraborty

5 Questions 11 Answers 0 Followers

Questions related from Amlan Chakraborty

What is the best way to block mouse MHC class II for in-vitro cultures?

I intend to block the MHC-II(I-A/I-E) on mouse antigen presenting cells in-vitro. Which technique will be the best?

28 June 2017 6,067 3 View

Can anyone suggest a protocol to get superparamagnetic iron oxide nanoparticles (SPIONS) of the size less than 40 nm measured using DLS method?

I could get as low as 100 nm with the size determination using a Malvern zetasizer. A co-precipitation method is preferable for me. Also I need suggestions for controlling the size like pH, flow...

07 March 2016 1,537 6 View

Is my gene amplifying?

I have a gene(wild type) cloned in pBAD18 Kanamycin resistant plasmid. The gene is 2185 bp. And the plasmid is 5437 bp. I wanted to insert a 6X His tag at the C-terminal end of the protein of this...

31 March 2015 3,646 11 View

How do you tag a mixture of Phytochemicals with 99mTc?

I want to tag a formulation made of multiple phytochemicals which constitutes flavonoids and alkaloids with 99mTc. I will be using the 99mTc tagged formulation for gamma imaging on mice or New...

30 July 2014 6,673 2 View

How to detect bacteria present in human blood directly?

I want to detect bacteria from blood directly. The same has been possible very easily from stool samples but how will it be possible from blood?

14 July 2014 2,515 10 View