What's wrong with nuclear markers DAPI or Hoechst? Any good antibody towards transcription factor active in epithelial cells will probably do the job...
If you want to look for dividing cells, then Phospho histone H3 should be good one...in other case if the cells are in the cell cycle then use PCNA.
Mari Mari Palgi : thank u so much for answering my question.i am interested in transdifferentiation . i am GTRACing some cells. if i gtrace some cell types, i cannot use GFP and RFP anymore as they are included in gtrace. i am interested in seeing whether they are transdifferentiated into epithelial cells.so, if i use some primary antibody localised only to nucleus then i can use secondary antibody having RFP or GFP.
Sa Chen : thank you so much. but i need epithelial cell specific nuclear localised primary antibody.
Hi Jayesh,
Could you possibly use antibodies against epithelial markers that are not nuclear-localized for GTRACE? I ask because most of the reliable epithelial specific antibodies that I have used, e.g. those against the septate junction protein Coracle, tend to be membrane specific.
A possible alternative would be using FISH to hybridize transcripts for epithelial markers. It wouldn't be quite as easy or as sensitive for GTRACE-ing, but might get the job done.
Shaad M Ahmad: thank u so much.yeah, i can use some membrane specific markers also.i will try doing it. if not, i will go for FISH.
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