How to set up a weighted sample in meta-analysis?

I want to run a meta-analysis and I have to set up a weighted sample for each subsample from my study. For example a paper reports four different fiscal multipliers estimates (these are the...

06 February 2021 5,031 3 View

[Sand Lizards tracked with VHF telemetry] How do I analyze the results of my home range estimations properly?

Hello, I just recently started working with the different types of home range estimations and have a few questions about this and that. Maybe you could help me out with that?! My study animals are...

03 February 2021 5,573 3 View

How do I calculate the expansion factor and the distortion between pre- and postexpansion 3D images?

Hello all I am performing expansion microscopy experiments and I need to calculate the expansion factor and the distortion during the expansion.My data is 3D. I tried to use Elastix software but...

10 January 2021 3,109 4 View

What statistical analyses can I use for non-normal data overtly recorded with a 3 point scale and underlyingly recorded along a 0-100 scale?

I am struggling to understand what method of statistical analysis would work best for my data. I have a small sample of repeated measures data (15 participants in total) and I've asked them to...

09 January 2021 8,118 4 View

Public databases on insect morphometric characteristics?

Could anyone suggest any online sources (databases, dedicated websites, etc.) on insect morphometric characteristics, please? I'm particularly interested in insect mouthparts and tarsi...

04 October 2020 9,651 5 View

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03 September 2020 8,865 2 View

How can I conduct home-made thin film interference experiment?

Hello! I need to conduct an experiment, where I should measure thicknesses of different thin films (oil on the water surface, soap bubble etc.). Due to quarantine I have no opportunity to use any...

20 April 2020 9,509 2 View

How to obtain the permission to use a Comorbidity Score in a non-profit study?

I would like to want to know how to obtain the permission to use for one / all of the following Comorbidity Scores? If you know the contact e-mail or the respective on-line page. Thank...

28 April 2019 9,431 1 View

Best sequence editing software?

I've been using BioEdit for a decade but since it has no support and more like abandonware now I'd like to switch to a new sequence editing software which is being actively maintained. I would...

03 February 2019 7,284 6 View

How to solve problem regarding invitrogen exosome isolation kit?How to successfully characterise exosomes by western blotting technique?

29 June 2018 4,485 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View