Can I compare two mean scores of the same instrument, in the same sample? (Paired t-test)?

Hello everyone, I have scores on different scales of a sample of children, and I want to compare the mean scores on the different scales of this sample. For instance, my hypothesis is that...

10 December 2020 2,278 5 View

Protocol for proliferation of CD4+ murine T-cells?

23 July 2019 3,586 7 View

How to dissolve Dasatinib in order to go in vivo?

Dasatinib is an inhibitor of tyrosine kinases. I would like to test the effect of Dasatinib in vivo on my T cells. However, Dasatinib has a very low solubility (0.5mg/ml) with PBS, which is...

21 July 2019 3,056 3 View

Lysing of cell to detect antibody internalization?

07 April 2019 3,293 10 View

Fix 7-AAD staining?

Hi, I would like to make a viability staining using 7-AAD the day before the measurement; Is it possible to fix the cells? If yes, what do you suggest? Thank you!

01 April 2019 4,376 2 View

OT-I Rag1-/- genotyping protocol?

Dear all, Does anyone have a protocol to provide me to genotype OT-I Rag1-/- mouse? I have taken a look to OT-I and Rag1-/- genotyping protocols and I would need 4 primers for Tcra, 4 for Tcrb and...

10 December 2018 2,449 2 View

I need to measure the respiration rates in insects larvae. Do you have any suggestions about a feasible and not expensive equipment that can be used?

I know that there are avaialble some respiration chambers for insects but I was wondering if chambers used for small fruites can be adequate for the scope.

15 October 2017 3,205 2 View

What statistical analysis should I use for hierarchical questions data?

16 November 2015 9,532 4 View

Is it possible to change the orientation of hydrogen atoms of the ligand and protein residues? If yes, how is it possible by using Gromacs 5.0.4?

Dear users, I would like to change the orientation of an hydrogen of a D-serine (my ligand) and of a serine (as protein residue). How is it possible by using Gromacs 5.0.4? Thank you

12 September 2015 6,906 5 View

Could you please recommend me a manufacturer/ vendor of the GLAD(Glancing Angle Deposition) system?

Ideally we would like to buy a PVD equipment that can be used for the layer by layer and columnar growth.

09 June 2015 9,952 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View