Greiss reagent for NO detection?

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Yurii V Geletii

Greiss reagent does not exist. The correct spelling is Griess. Griess assay is designed to measure nitrite but not NO. Under certain conditions NO is oxidized by O2 to form exclusively nitrite, which can be detected by Griess reagent. The protocol to measure nitrite is pretty simple and described in numerous papers

Frank T. Edelmann

Dear Stefania Pasqualini thank you for your interesting technical question. In this context please have a look at the following relevant article:

Measurement of Nitric Oxide Production in Biological Systems by Using Griess Reaction Assay

Article Measurement of Nitric Oxide Production in Biological Systems...

This paper is freely available as public full text on ResearchGate.

P.S. You can easily edit your question and replace "Greiss" by "Griess". Just click on the down arrow next to your name and edit your original question.

Dear everybody, could you advide me a procedure to recover lactic acid from PLA?

Can effect direction be used for evidence other than interventions?

Does anyone have a copy of Hensley's 1974 paper on public speaking anxiety?

Are there any cons in using immobilization and running buffers at pH 5.5 in SPR?

Are there any constraints to EEG paradigms for using hierarchical Bayesian models?

Which blood parameters would you analyze to evaluate the oxidative status in broilers as a consequence of microalgae supplementation?

LightCycler 480 software: how to export ct value and fluorescence of a PCR in excel format?

Can I compare two mean scores of the same instrument, in the same sample? (Paired t-test)?

Cff strains and Cff naturally infected seeds?

Is there a competition between cortico-cortical connectivity (imaginary coherence) measured at different frequencies?

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument​?

Interested in a SCOPUS collaboration?

Interested in a SCOPUS collaboration?

What is the problem with these tissue culture plants?

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?