We are starting soon an in vivo trial with broilers fed diets supplemented with microalgae and insect full fat larave. We will collect blood samples and I'd like to have your suggestions about the best parameters to be analysed on blood to evaluate the oxidative and metabolic status
Dear Dr Stefania Colombini . See the following useful RG link: Article Dietary supplementation with DHA-rich microalgae improves pe...
Kindly check also the following useful RG link: Article Influence of dietary combinations of Amphora coffeaeformis w...
Dear Stefania :
You can analyze:
- Glutathione peroxidase (GPx): main biological role is to protect the organism from oxidative damage
- Superoxide dismutase (SOD): out-competes damaging reactions of superoxide, thus protecting the cell from superoxide toxicity.
- Catalase: very important enzyme in protecting the cell from oxidative damage by reactive oxygen species
- Total antioxidant capacity (TAC): indicate the ability to counteract oxidative stress-induced damage in cells. Also, used to provide insights into the development and treatment of oxidative-stress related disorders.
Also check please the following good RG link: Article Effect of Dietary Microalgae on Growth Performance, Profiles...
Oxidative stress products such as Malondialdehyde, Protein Carbonyls, Total Antioxidant Capacity using FRAP Assay. Antioxidant enzymes activities such as Superoxide dismutase, catalase activities, and glutathione concentration. We have determined some of these in blood of rabbit models, so it could also be possible in broilers.
MDA,TAoC,Superoxide dismutase, catalase activities, and glutathione concentration
You can analyse the following parameters in broilers liver and/or in broilers meat: Malone dialdehye(MDA), Free fatty acid (FFA) and Peroxide value (PV)
When MDA, FFA and PV are in high concentration in meat or liver this means that the oxidative stress is high
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