Can anyone share how they label monocytes with CellTracker Orange(CMRA)?

Sharon Lee @Sharon-Lee-29

19 December 2017 0 4K Report

I use IMDM as staining buffer (is this ok?),but lose 60% cells after staining.

Also, any idea/comment on why we should avoid amine-containing or thiol-containing buffers as the staining buffer?

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