16 Questions 5 Answers 0 Followers
Questions related from Sharon Lee
I used NucBlue Live from ThermoFisher (https://www.thermofisher.com/sg/en/home/references/protocols/cell-and-tissue-analysis/protocols/nucblue-live-readyprobes-protocol.html). I found that this...
07 May 2018 1,428 1 View
These 'secretion-inhibited' cancer cells would then be co-cultured together with myeloid cells so I am hoping the environment would not be toxic to both cell types.
24 April 2018 7,765 8 View
I use IMDM as staining buffer (is this ok?),but lose 60% cells after staining. Also, any idea/comment on why we should avoid amine-containing or thiol-containing buffers as the staining buffer?
19 December 2017 4,015 0 View
Wondering how long these cells can survive in culture, and if cells have special requirements.
05 June 2017 3,767 1 View
Would T cells kill monocytes? Would monocytes stimulate/activate T cells?
06 April 2017 6,475 5 View
Is 4 hours too long? Would the MFI signal decrease by more than 50% of the expected value?
18 January 2017 3,151 4 View
After centrifuging in a v-bottom 96-well, at 2000rpm (800G), 3min, 4deg, (volume about 180ul) I added 180ul buffer without breaking up the pellet, and then centrifuged the cells again. (i.e i...
18 January 2017 975 1 View
Observed some evaporation of the culture media...but inside of incubator felt mostly warm (i.e. 37deg).
14 December 2016 2,148 2 View
I accidentally spilled some sterile milliQ water on my cells that were already in culture media. I immediately removed all the media and replaced it with fresh media. In my case, sholud I be...
13 December 2016 5,751 5 View
For different donors, we observed the upregulated expression of immune checkpoint ligands on monocytes when they were cultured with only CD8 T cells (both antigen-specific and non-specific CD8 T...
20 November 2016 1,985 3 View
Antibody used to stain for expression of inducible surface protein.This is a primary conjugated antibody for flow cytometry. Fluorophore: Brilliant Violet 711 (Similar to Qdot711); Considered to...
19 November 2016 442 4 View
We suspect that the 'ferocious' immune response that we observe is due to the presence of a conjugated ligand.
14 November 2016 1,917 3 View
IL-2 is provided in powder form. To prepare, I dilute in millipore water and make small aliquots. When preparing cell culture media, I add the IL-2 stock to the media. Then, I filter the...
13 November 2016 8,768 2 View
Heard some researchers have always purchased RPMI (no L-glutamine), and only added L-glutamine when preparing the complete cell culture media. Wondering if this has to do with the...
11 November 2016 3,677 3 View
Cell types: HepG2, Monocytes, T cells Note: Collected cells will be stained for surface antigens, and flow cytometry is used to measure for expression levels.
08 November 2016 6,553 3 View
Particularly, the media is AIM-V (see link) by Gibco, which the company claims can be used without serum to culture certain cell types....
26 October 2016 3,701 1 View