I read the protocol described in " Quantitative assay of senescence-associated B-galactosidase activity in mammalian cell extracts" (Gary, 2005) but the preparation of the lysis buffer and other buffers seems complicated and expensive. Has anyone tried using a different lysis method? or any other minor modifications? please share your experiences.

Also, if anyone shares a protocol (easier, cheaper) to measure senescence, I would be very grateful.

Thanks