Reccomendations for Core/Whole Genome MST and Core Genome SNP-Based Typing?

Dear all, I am analyzing WGS sequences of Enterobacteriaceae. However, I do not have any experience in software based on OSX or Linux. Could you recommend me user-friendly software (better free)...

31 December 2019 9,663 2 View

Could I apply Greentaq Master Mix for BOX-PCR or (GTG)5-PCR?

I'm looking for apply GreenTaq Master Mix in order to detect bacterial strain fingerprintings using (GTG)5-PCR and BOX-PCR. It is possible to apply it? Thank you for your thoughts!

15 April 2018 964 3 View

Could I apply Greentaq Master Mix for BOX-PCR or (GTG)5-PCR?

I'm looking for apply GreenTaq Master Mix in order to detect bacterial strain fingerprintings using (GTG)5-PCR and BOX-PCR. It is possible to apply it? Thank you for your thoughts!

03 April 2018 5,833 3 View

Any reccomendation of 16S rRNA standard for qPCR?

I'm looking for a sequence or organism that I could use as a standard for 16S rRNA quantification by qPCR. And how I could validate the LD and LC. I'm using TaqMan probes. Thank you in advance.

20 March 2018 6,646 5 View

Any reccomendation of 16S rRNA standard for qPCR?

I'm looking for a sequence or organism that I could use as a standard for 16S rRNA quantification by qPCR. And how I could validate the LD and LC. I'm using TaqMan probes. Thank you in advance.

02 March 2018 7,283 5 View

What is the best reference miRNA in qPCR of miRNA when working with human cancer cell lines?

I am having some trouble with RNU6B as endogenous control for several ovarian cancer cell lines. Could you recommend me other stable reference miRNAs that have worked in your experimental approaches?

20 June 2013 890 19 View

Which mask is appropriate in COVID-19 pandemic?

Hello everyone, I hope you are well. In the literature it is mentioned that medical masks are made of non-woven materials, in general the main component is polypropylene. Masks for medical use...

01 January 1970 7,144 12 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View