What causes the white coloration in shelf fungi?

During late June of 2020, I had picked some relatively fresh Schizophyllum commune. By the time I got around to examining them, they had become dried and shriveled. I placed the point of...

02 December 2020 4,193 3 View

Is there a proper citation for the human genome assemblies?

I am currently writing up results from a study that utilized the hg19 [Genome Reference Consortium Human Build 37 (GRCh37)] for analysis and I have not been able to identify a way to cite this...

24 November 2020 9,960 2 View

Professional Engineer's Code of Ethics: What & How To Assure Compliance?

Historically, the subject question has been met mainly with "After The Fact" assertions. Yet when one learns of the infrastructure failures . . .bridges, tunnels, pipelines, highways. . . it...

11 November 2020 3,574 3 View

Parsing error in autodock vina?

I'm trying to use autodock vina, and I received the following error for the ligand file when running the configuration: "Parse error on line 26 in file "15.pdbqt": Unknown or inappropriate...

21 October 2020 7,245 1 View

How do we use adjusted prevalence rate (aPR) in a meta-analysis?

Hi, I am doing a meta-analysis using Review manager determining association between increase in pollutant concentration and the risk of a particular disease. Majority of outcomes are reported as...

20 October 2020 10,012 3 View

How can I calculate prevalence ratio on Stata?

Hello, Our study aimed to estimate the stress prevalence among health professionals who works on pre-hospital services. The dependent variable was the presence or absence of stress (dichotomic...

16 August 2020 9,595 5 View

Q1. What changes have been observed in the professional lives of our CE graduates ? Q2. What CE academic programs have developed?

In June 1995, the culmination of some 3 years or so of preparation, led by professor Dr. Jim Yao, Texas A&M, resulted in an "ASCE Education Conference" in Denver, CO. At the end of that...

12 August 2020 1,069 3 View

How can I delete a document and replace it with proper text?

I have added a new document to my documents but found it was the wrong file. How do I delete the current document so I can replace it with correct one.?

03 August 2020 292 1 View

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16 June 2020 2,168 1 View

Native English speakers and/or PIV intermediates: Could you check the wording of my PIV tool...?

Hello everyone, I have made a free PIV tool for Matlab in English, but I am not a native speaker. I would like to make the tool as clear as possible because it is used quite often. Could someone...

09 June 2020 5,449 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Consensus sequence from ClustalX?

I am doing multiple sequence alignment to design primer for my gene. Can anyone tell me how to select the consensus sequence after alignment. I am using ClustalX for the alignment.

04 February 2021 8,872 2 View

Alternatives to .45 um filter to isolate MS2 from lysed E. coli?

Hello, I am looking for alternatives or cheaper materials to isolate out MS2 from E. coli following lysis via chloroform. Some literature I have reviewed uses a .45 um nanopore filter following...

02 February 2021 807 3 View

What is the suitable buffer for this protein ?

I am trying to find a suitable buffer for storage of a His-tag purified protein. The amino acid sequence is pasted below. Can anyone help please? MMSFVSLLLV SILFPAIQAK QFTKCELPQV LKDMDGFGGI...

01 February 2021 5,216 4 View

What are some good protocols for native outer membrane protein extraction from E. coli?

I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...

25 January 2021 5,736 3 View

Where can I find the receptor binding domain (amino acid sequence) of ALL pathogenic viruses?

I want to compare the RBD of all pathogenic viruses? Is there a database or manuscript that anyone knows of where I can access these?

25 January 2021 3,843 4 View

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

24 January 2021 9,987 3 View