Reccomendations for Core/Whole Genome MST and Core Genome SNP-Based Typing?

More William Calero-Cáceres's questions See All

Could I apply Greentaq Master Mix for BOX-PCR or (GTG)5-PCR?

I'm looking for apply GreenTaq Master Mix in order to detect bacterial strain fingerprintings using (GTG)5-PCR and BOX-PCR. It is possible to apply it? Thank you for your thoughts!

15 April 2018 964 3 View

Could I apply Greentaq Master Mix for BOX-PCR or (GTG)5-PCR?

I'm looking for apply GreenTaq Master Mix in order to detect bacterial strain fingerprintings using (GTG)5-PCR and BOX-PCR. It is possible to apply it? Thank you for your thoughts!

03 April 2018 5,833 3 View

Any reccomendation of 16S rRNA standard for qPCR?

I'm looking for a sequence or organism that I could use as a standard for 16S rRNA quantification by qPCR. And how I could validate the LD and LC. I'm using TaqMan probes. Thank you in advance.

20 March 2018 6,646 5 View

Any reccomendation of 16S rRNA standard for qPCR?

I'm looking for a sequence or organism that I could use as a standard for 16S rRNA quantification by qPCR. And how I could validate the LD and LC. I'm using TaqMan probes. Thank you in advance.

02 March 2018 7,283 5 View

Can anyone recommend me free software for making and editing maps?

I'd like to hear experiences about which one is best for good quality map for scientific publication. I should like to draw a river basin and detail the sampling points. Thanks for your thoughts!

05 June 2015 1,932 18 View

What is the best reference miRNA in qPCR of miRNA when working with human cancer cell lines?

I am having some trouble with RNU6B as endogenous control for several ovarian cancer cell lines. Could you recommend me other stable reference miRNAs that have worked in your experimental approaches?

20 June 2013 890 19 View

Which mask is appropriate in COVID-19 pandemic?

Hello everyone, I hope you are well. In the literature it is mentioned that medical masks are made of non-woven materials, in general the main component is polypropylene. Masks for medical use...

01 January 1970 7,144 12 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Rex Frimpong Anane

Hi William Calero-Cáceres,

You can this article: Article Whole genome and core genome multilocus sequence typing and ...

for Core/Whole Genome MST and Core Genome SNP-Based Typing analysis pipeline and tools.

Hamidah Ghani

You can Scan contig files against traditional PubMLST typing schemes (https://github.com/tseemann/mlst). Ask someone that familiar with bioinformatics to help