Hello.
I work in a cancer immunology group. We need to deplete CD45 (.1 /.2) OT-1 cells previously transferred to CD45 (.1) mice, with aCD45.2 depletion antibody. But we have doubts about the dose to use. We usually deplete CD8+ T cells with 60ug of aCD8 depletion antibody (YTS169.4) but this time we need to deplete a smaller number of cells and maybe 60ug of aCD45.2 is an excess. In their work, Laura Mackay's team uses about 10ug of the antibody aThy1.1 (HIS57) to deplete CD8+ T cells Thy1.1+.
Beforehand thank you very much.
Hello, I suppose you are more experienced than me in doing adoptive T cell transfer. May I ask one question. I have transferred CD45 (.1 /.2) OT-1 cells into B16-OVA melanoma-bearing CD45 (.1) mice, but one week later I can not detect any transferred T cells. The transferred T cells were isolated, stimulated by plate-bound CD3/CD28 and then expanded by IL-2. We did not give any treatment to the recipient mice. what problem do you think it could be.
Beforehand thank you very much
I am so sorry for such a delay, I had not reviewed Researgate in many weeks.
Could you solve your problems ?. We have performed similar experiments and we have not had that problem, so I am not sure what it could be. One question, does your cell therapy promote tumor eradication?
I have seen that some researchers use treatments with IL-7 / IL-15 to favor memory phenotypes that last over time favoring the antitumor response.
It could also be that your B16 cells have lost the expression of OVA, do they have a probe to follow the expression of OVA ?.
I stay tuned in case you need help.
- Badges
- Science method
- Similar topics
- Mathematics
- Statistics
- Statistical Modeling
- Analyses of Variance
- ANOVA