Hello

please read the reference in attachment , and visit this website http://www.cancerci.com/authors/instructions

even help you better , suggested that determine the type of cancer cell that you want to culture ( normal cell vs stem cell or what...) , and the sources of these cell ( human or animals )

Dear Professor Alkarim

Thanks for sharing your information and your great attached book and suggested website. I want to culture MC4-L2 cells ( a murine model of breast cancer).

Best regards

Dear Mahmud Delphan if you have purchased cell line from a certified cell factory  such as ATCC, you can fuond the data sheet on the web site. Usually cells were grown in T75 flasks in DMEM/F-12 with 15 mM HEPES buffer, L-glutamine, penicillin 100 μg/ml, streptomycin 100 μg/ml, 10% FBS (Gibco BRL, Life Technologies) and 10 nM Medroxy Progesterone Acetate (MPA) (Sigma Chemicals, Ontario, Canada).

Dear Dr. Conti,

Thanks for sharing your information.

Undoubtedly, your shared information will be helpful. Please tell me about why do we use MPA in MC4-L2 cell culture? Is it essential in MC4-L2 cell culture? Meanwhile,this is the first experience that I will start to culture MC4-L2 cells! It is also the first cancer cell culture that I myself will it. Hence, I really need to learn step-by-step method of cancer cell culture.

hello

Dear Mahmud

I hope read the reference in attachment accurately, will find an answer for your questions

Good luck

Hello

Dear Professor Alkarim,

Thanks for your reply.  Surely, your attached files will be helpful.

Best regards

There are many types of cancers ... You do not determine which type of cancer that you interst for ?

Breast cancer in mice which I interest .....