I have very high variation in MTT assay between the replicates.
I have a problem with dectection of human DNA using qPCR. I isolated DNA from human stem cells using DNA blood and Tissue kit from Quagen. Nanodrop measurements demonstrated 250 ng/ul...
07 August 2012 2,742 9 View
I am going to measure DNA concentration using spectrophotometer. Please advice how to rinse quartz cuvette between different DNA samples. Will ddH2Ol be sufficient, or EtOH (70% - 95%) is required?
06 July 2012 4,892 18 View
For those who perform HET-CAM assays - how do you convince hatchery owners to cooperate with you in providing hundreds of fertilized hen's eggs for the assay? We usually purchase eggs that were...
31 December 2011 1,266 0 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
02 March 2021 3,060 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
28 February 2021 9,936 2 View