I have very high variation in MTT assay between the replicates.
Dear all, Does anybody have a pdf of this paper? Wood, G.R. and Beeston, J.W., 1986. A late Permian lycopod cone Skilliostrobus sp. cf. S. australis Ash 1979 from Queensland. Geological Survey of...
07 August 2024 4,857 2 View
Liu Zhaosheng. 2003. Triassic and Jurassic sporopollen assemblage from the Kuqa Depression,Tarim Basin of Xinjiang,NW China. Palaeontologia Sinica, New Series A,no. 14(Whole no. 190)....
28 July 2024 6,390 1 View
I have ChemDraw 22.2.0 and I want to draw PC70BM but I just found C60 and less in the templates so, any one can help me ?
18 June 2024 3,098 3 View
In my current study I'm measuring cultural diversity in intercultural coaching dyads by calculating cultural distance (Kogut-Singh Index, 1988) between coach and coachee in each intercultural...
03 June 2024 9,990 1 View
Как найти свой Researcher ID?
30 May 2024 6,369 0 View
How should I prepare electrospun fibers for BET analysis? How much (cm x cm) should I cut to obtain reliable results? What about degassing? I have PLA sample, 40 C will be enough? Thank you
23 April 2024 7,364 2 View
Dear colleagues, Does anybody by chance have a pdf of this paper? Coquel, R., & Brousmiche Delcambre, C. (1996). Comparaisons spores in situ-spores dispersées chez quelques Equisétales,...
19 March 2024 8,258 3 View
In my experiment, one group didn't have Cq when a use a specific primers to specific gene. But another groups was ok. How can i represent this results on the graph? I need to put Cq = 0 or Cq = 40?
17 March 2024 6,789 0 View
Hi everyone, could you recommend any article on CD marker profile changes and their biological significance due to this change? I have been looking for something but can't find anything. In my...
10 March 2024 3,702 0 View
I successfully amplified fungal ITS from soil samples, however after running the purified PCR products in an agarose gel they are barely visible and don't look like defined bands but rather...
10 March 2024 8,377 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View