Can anyone recommend an HIF1 reporter that works in mice?

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Has anyone used a non-biliary hepatocyte marker for IHC that works on human frozen tissue?

Can anyone recommend an hepatocyte antibody for IHC that they have used on frozen human liver? There are markers that will stain hepatocytes and cholangiocytes, but I would like to stain only...

11 December 2019 7,603 2 View

Why am I getting inconsistencies in my PCR duplicates?

I am amplifying a ~9kb mitochondrial product and keep getting inconsistencies in technical duplicates. In the attached image, I have 6 samples (#1-6; 1ng template from laser-captured tissue) #7's...

01 February 2019 2,821 12 View

Why am I losing DNA using QIAamp DNA Micro kit?

I'm having an issue that is possibly due to the QIAamp DNA extraction? I am extracting DNA from laser capture microdissection (LCM) of frozen section material. What I have been doing is cutting...

01 February 2019 7,761 9 View

Can someone recommend a protocol for extracting RNA from stained laser microdissected samples?

I'm aiming to extract RNA from laser microdissected samples. Can anyone offer advice or point me to a protocol that works? I want to be able to dissect stained (IHC) cells from a section (I've...

07 August 2013 8,161 2 View

Cell fractionation for Western Blot - protocol

I've been looking at protocols to do cell fractionation. I want to be able to do Western Blots on both the cytosolic and nuclear fractions. Currently I'm not seeing any of my protein in the...

01 February 2013 3,996 11 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

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What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

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26 February 2021 7,129 3 View

Is IMPACT FACTOR justified in case of Taxonomy?

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26 February 2021 5,851 1 View

Anna Habryka

I think, You could use a carbon anhydrase IX (CAIX, for transcript or protein analysis). It is good positive control for hypoxia experiments, both in human and mouse cells. It is directly control by HIF1. You can also use GLUT1 and VEGFA. Fixed cells can be also stained with pimonidazol (protocols are easy to find in internet). Good luck :)

Adam M. Passman

thanks for the advice!