Can anyone recommend an alternative for 1-methyl-3-isobutylxanthine Rat BM-MSC Adipogenesis differentiation media?

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Eric R Hugo

You might consider caffeine or theophylline (I would guess around 1 mM). Another possibility might be forskolin which directly activates adenylate cyclase although I have no idea of the concentration or its half-life in medium. Just out of curiosity why are you trying to avoid IBMX? I have a fairly potent adipogenesis medium recipe I use that has some additions to the typical cocktail out there if you are having induction issues.

Krishna Ambilwade

Hello Guys

I am working on adipogenesis in mouse embryo fibroblast cells (MEF)

in process of differentiation cells come off easily from tissue culture plate

Please suggest me what coating you use on tissue culture plates (specifically for MEF cell lines)