Eric R Hugo

2 Questions 18 Answers 0 Followers

Questions related from Eric R Hugo

Will cholesterol supplimentation of CD hybridoma medium harm non NS0-derived hybridomas?

I have some hybridomas that were generated for us commercially and I do not have too many details on what mice or myeloma was used to generate them. We would like to adapt them to CD Hybridoma...

14 April 2017 1,296 1 View

Has anyone had a problem with getting a pcr reaction to work with Phusion HS II polymerase that works just fine with regular Taq (DreamTaq)?

Having problems with a RT-PCR reaction that works fine with regular Taq but gives absolutely no product with Phusion Hot Start II from Thermo. I replaced my dNTP stock with new material, tried...

31 March 2017 7,408 5 View