Has anyone had a problem with getting a pcr reaction to work with Phusion HS II polymerase that works just fine with regular Taq (DreamTaq)?

Having problems with a RT-PCR reaction that works fine with regular Taq but gives absolutely no product with Phusion Hot Start II from Thermo.  I replaced my dNTP stock with new material, tried...

31 March 2017 7,393 5 View