I had been facing a similar problem with Promega Kit. But now I heat the nuclease free water before elution & incubate the water in the column before elution for 3-5 mins. This worked for me in getting better yield.
Hello Essak, thankyou for the advice, but I have already tried waiting for 5 min before elution from the colum without improving the yield significantly.
Any other recommendation?
Back in the old days (1990's) we would use dialysis tubing and just cut the band of interest out of the gel and place it in a short segment of dialysis tubing between tubing clamps. Then place the tubing in the gel apparatus and use electrophoresis to migrate the DNA out of the gel fragment. You can also use a low % gel and melt the agarose, dilute it and just precipitate the DNA depending on how pure you need it.
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