Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Are the molecular biology reagents of HiMedia ( kits,pcr and electrophoresis products) as good as products of NEB, Sigma and Invitrogen?

HiMedia seems to be cheaper than the rest.Anybody who has used it for molecular biology experiments, how is the quality?

26 September 2019 7,010 3 View

Annealing temperature in RT PCR - Positive control differes from target gene?

The primer annealing temperature , after the gradient PCR analysis, for the target gene differs from the optimal annealing temperature in housekeeping gene (positive control). What to do ? The...

02 July 2019 810 3 View

What statistical test can i use on my data?

I am researching the relationship between animal biodiversity and physics-chemical properties of a clean and polluted burrow dyke. I have the species abundance for both burrow dykes, as well as...

26 June 2019 569 2 View

Segregation coefficient of self-compacting concrete and small superplasticizer overlaps?

What do you think how important, for segregation coefficient of SCC, could be very small superplasticizer overlaps during the process of making SCC? I made some observations in my work: Some...

04 May 2019 6,951 4 View

What policies may bring about sustainable reform and development?

Social disparities rise amid volatile economic and political times today. What may be constructive efforts in policy form to start resolving issues that cause such state of affairs?

16 March 2019 1,086 4 View

How can I improved the power (accuracy) of my Deep Learning Model?

Hey everyone, me and my colleagues are programming a Deep Learning Model with tensorflow. Our goal is it to predict chemical toxicity. Our input are chemical drugs in SMILE-Strucuture. So, how...

07 June 2018 7,920 3 View

What are uses of waste in geotechnical engineering?

waste use in improving soil engineering specification such as disaster waste, cement waste and others

02 August 2017 8,663 16 View

Dummy/ real data Request

02 July 2017 5,438 2 View

Do you know a scale for entrepreneurial learning?

Looking for a scale for entrepreneurial learning types (individual vs. collective learning, exploratory vs. exploitative, intuitive vs. sense making learning

20 September 2016 4,647 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View