Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Ferret PBMC Fc block?

Hi all, I'm going to run Flow Cytometery on ferret PBMCs but can't find an Fc block for ferret PBMCs. Does that even exist? Do I need ferret-specific Fc blocking antibodies or can I use:...

18 January 2021 4,504 5 View

Platelet Counting on Flow Cytometry?

Hi, Does anyone have a method for counting platelets on flow cytometry (cytometer=Cytoflex) without the need of using TRUcount beads or reference beads? Is it possible or do I need to purchase...

23 September 2020 3,509 1 View

What is the best bibliographic output style for preparing a CV?

I am looking for an output style in Endnote to list publications in one's CV. These would have to be be listed in reverese chronological order. Recommendations and opinions welcome.

23 March 2020 1,228 4 View

Anyone using stereo trail cameras (camera traps) linked to gather morphometric data from cryptic species?

Looking to connect with any research, papers or groups using remotely triggered stereo cameras to create 3 dimensional pictures of wildlife and gather data on cryptic species. Wanting to set up a...

01 February 2020 940 3 View

IHC-P: Bone falling off slides?

Hello everyone, I am performing IHC for von Willebrand Factor on femurs and tibiae of 22-week-old SD rats. The bones are decalcified in EDTA and paraffin embedded. After antigen retrieval,...

19 November 2019 6,567 12 View

How can I control the evaporation of water from my ceramic samples?

I work with ceramic aqueous suspensions which I pour into a 3D-printed mold. My current problem is the fact that water evaporates too quickly from my samples, causing bubbles and other...

24 June 2019 1,988 4 View

Finding EA(energy absorption)?

Hello every one, I am new to abaqus. How do I find energy indicators like, EA(energy absorption), Mean Crush Force (MCF) or Crush Force Efficiency (CFE) in a result section for a buckling process...

05 May 2019 5,193 3 View

Is my technique at fault while loading gels for electrophoresis?

I recently completed a gradient PCR but my bands were much less than satisfactory. I have a nagging feeling that my pipetting technique is at fault when loading. I use standard forward pipetting...

17 December 2018 1,100 4 View

What am I doing wrong - extracting a sapogenin for NMR?

I use hydrochloric acid to release a steroidal sapogenin from its saponin, then neutralise it with sodium bicarbonate. I am confident the hydrolysis works because the mixture no longer produces a...

03 June 2018 3,814 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View